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KMID : 0361020210640040232
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Korean Journal of Otolaryngology - Head and Neck Surgery
2021 Volume.64 No. 4 p.232 ~ p.239
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Ginsenoside Rb1 Attenuates TGF-β1-Induced MUC4/5AC Expression and Epithelial-Mesenchymal Transition in Human Airway Epithelial Cells
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Choi Tae-Yeong
Kim Joon-Hee
Jo Soo-Yeon
Lee Sang-Jae
Na Hyung-Gyun
Choi Yoon-Seok
Song Si-Youn
Kim Yong-Dae
Bae Chang-Hoon
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Abstract
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Background and Objectives : Ginsenoside Rb1 is the main metabolite of Panax ginseng. Itis known to have many beneficial properties including anti-inflammatory, antitumoral and antioxidanteffects. However, the therapeutic effects of ginenoside Rb1 on inflammatory airwaydiseases have not been elucidated. Therefore, we investigated the effects of ginsenoside Rb1 onthe TGF-β1-induced mucin gene expression and epithelial-mesenchymal transition (EMT) inhuman airway epithelial cells.
Materials and Method : We evaluated the effects of ginsenoside Rb1 on the changes of MUC4,MUC5AC, occludin, claudin 4, claudin 18, neural (N)-cadherin, and epithelial (E)-cadherin expressionby TGF-β1 in NCI-H292 cells using reverse, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot.
Results : TGF-β1 significantly increased MUC4/5AC expression. Rb1 inhibited TGF-β1- inducedMUC4/5AC expression. In addition, TGF-β1 significantly attenuated occludin, claudin 18,and E-cadherin expressions but induced claudin 4 and N-cadherin expressions. On the otherhand, Rb1 reversed changes in the TGF-β1- mediated expressions of cell junction molecules.
Conclusion : These results suggest that ginsenoside Rb1 attenuates TGF-β1-induced MUC4/5AC expressions and EMT in the human airway epithelial cells. These findings are importantdata demonstrating the potential of ginsenoside Rb1 as a therapeutic agent for inflammatoryairway diseases
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KEYWORD
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Airway epithelial cells, Epithelial-mesenchymal transition, Ginsenoside Rb1, MUC4, MUC5AC
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